@article {591846,
	title = {STENOFOLIA recruits TOPLESS to repress ASYMMETRIC LEAVES2 at the leaf margin and promote leaf blade outgrowth in Medicago truncatula},
	journal = {Plant Cell},
	volume = {26},
	number = {2},
	year = {2014},
	note = {<p>Zhang, FeiWang, YeweiLi, GuifenTang, YuhongKramer, Elena MTadege, MillionengResearch Support, Non-U.S. Gov{\textquoteright}tResearch Support, U.S. Gov{\textquoteright}t, Non-P.H.S.2014/03/04 06:00Plant Cell. 2014 Feb;26(2):650-64. doi: 10.1105/tpc.113.121947. Epub 2014 Feb 28.</p>},
	month = {Feb},
	pages = {650-64},
	abstract = {<p>The Medicago truncatula WUSCHEL-related homeobox (WOX) gene, STENOFOLIA (STF), plays a key role in leaf blade outgrowth by promoting cell proliferation at the adaxial-abaxial junction. STF functions primarily as a transcriptional repressor, but the underlying molecular mechanism is unknown. Here, we report the identification of a protein interaction partner and a direct target, shedding light on the mechanism of STF function. Two highly conserved motifs in the C-terminal domain of STF, the WUSCHEL (WUS) box and the STF box, cooperatively recruit TOPLESS (Mt-TPL) family corepressors, and this recruitment is required for STF function, as deletion of these two domains (STFdel) impaired blade outgrowth whereas fusing Mt-TPL to STFdel restored function. The homeodomain motif is required for direct repression of ASYMMETRIC LEAVES2 (Mt-AS2), silencing of which partially rescues the stf mutant phenotype. STF and LAMINALESS1 (LAM1) are functional orthologs. A single amino acid (Asn to Ile) substitution in the homeodomain abolished the repression of Mt-AS2 and STF{\textquoteright}s ability to complement the lam1 mutant of Nicotiana sylvestris. Our data together support a model in which STF recruits corepressors to transcriptionally repress its targets during leaf blade morphogenesis. We propose that recruitment of TPL/TPL-related proteins may be a common mechanism in the repressive function of modern/WUS clade WOX genes.</p>},
	keywords = {Amino Acid Sequence, Conserved Sequence, Gene Silencing, Medicago truncatula/*growth \& development/*metabolism, Molecular Sequence Data, Phenotype, Plant Leaves/*anatomy \& histology/*growth \& development, Plant Proteins/chemistry/*metabolism, Promoter Regions, Genetic/genetics, Protein Binding, Protein Structure, Tertiary, Repressor Proteins/*metabolism, Structure-Activity Relationship, Tobacco/metabolism},
	isbn = {1532-298X (Electronic)1040-4651 (Linking)},
	author = {Fei, Z. and Wang, Y. and R. Allen and Kramer, E. M. and Tadege, M.}
}
